The synthesized single strain oligonucleotides were separated by high performance liquid chromatography . the thermostable dna polymerase was used to catalyze snupe and the reactions of snupe for two y - snp loci were developed 利用耐热dna聚合酶催化单碱基引物延伸反应，建立2个y - snp的单碱基引物延伸反应。
